HI
I have not been able to get answers from PE on this question
so maybe some of you can help.
A customer of mine has run the products from a PCR reaction
on a polyacrylamide gel (4%) under denaturing conditions (UREA) and
TBE. His products are greater than 1000 b-- around 1500. when I run
the same reaction on a GeneScan gel, the products I see are less than
500b. the only difference I can see is that I denature in formamide
for 5 minutes at 96 degrees. Would this be sufficient to cause the
differences that we see?
He has run the reaction with both fluorescent-labeled primers
and unlabeled primers.
thanks in advance.
Deb G.
Deborah S. Grove, Ph.D.
Director of Research Projects
Nucleic Acid Facility
Biotechnology Institute
The Pennsylvania State University
210 Wartik Lab
University Park PA 16802
814 865 3332
http://www.lsc.psu.edu/stf/naf/home.html
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