As my question will make clear, I am a novice user of mass spec.
Presently I am working with yeast proeins. We cut a band of of a gel
and digest it with trypsin, make measurements of fragment masses via
MALDI and then use protein prospector to idetify the ORF. I understand
how all of this works (more or less).
But what happens in organisms with lots of introns? Does the program
predict the splicing sites well enough to get a reasonable approximation
to an ORF. That is, can I hope to do the same things with organisms
with lots of introns that I co with yeast?
Thank you for your help.
Danny Kohl
Daniel H. Kohl
Professor of Biology phone: (314) 935-5387
Washington University FAX: (314) 935-4432
St. Louis, MO 63130 email: kohl@biology.wustl.edu
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