RE>RE: methionine sulfoximine 5/10/2000
I wrote the following in response to David Andrews' reply:
"The hydrolysis idea is good, except that going back to methionine would require a reducing agent. But you got me thinking. Maybe hydrolysis and/or oxidation (H2O2?) would send it to methionine sulfone, which would shift the peak to another position! "
So, do the organic chemists in the group have input into that (perhaps naive) chemistry?
Vernon
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Date: 5/10/2000 3:46 PM
To: VERNON SHOUP
From: Andrews, David
this is really off-the-wall, but have you considered a mass spec method?...I
suspect if you used some sort of single-ion monitoring, you could quantitate
that stuff...another approach would be CZE, with fluorescence detection....
finally, can you hydrolyze the stuff back to methionine?...maybe you could
do an HCL treatment, and then just look for Met...
David W. Andrews, Ph. D.
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