You can take some of the resin,acid hydrolyze it and do A.A.A.It is not
going to be perfect,because some amino acids will be partially
destroyed,but it has saved me alot
of time.And,if you have a long or difficult peptide,you do the analysis
more than once.
especially when some difficult sequence is encountered.And,the simplest
check of all,is
the old-fashioned ninhydrin test!
Rebecca Ettling <ettlingr@musc.edu>@aecom.yu.edu> on 05/12/2000 08:38:19 AM
Please respond to ettlingr@musc.edu
Sent by: Association of Biomolecular Resource Facilities
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Subject: Pepsyn
O.K. It is time for another step in my peptide synthesis education:
I have run a PEB Synergy for 3 years. But now we have bought a new
synthesizer that does not have a conductivity trace (like the Synergy)
or any other feedback of how well the synthesis is going.
O.K. all you guru's out there . . . how do I know if what I am making
is any good? Do I really have to go all the way through the
synthesis/cleavage/MALDI routine, before I know whether or not I have
crap ?
As Always, thank you in advance !
Weather Inconsequential: Mid-may and the weathermen are already talking
about the "Heat Index" -- Sigh, spring must be over.
-- Rebecca P. Ettling
Biotechnology Resource Laboratory
Protein Sequencing and Peptide Synthesis Facility
Medical University of South Carolina
173 Ashley Avenue, Room 733D BSB
Charleston, SC 29403
Tel.: (843) 792-1271
Fax: (843) 792-1264
If I have seen farther than others, it is because I was standing on the
shoulders of giants.--Isaac Newton
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