I would use 1 M TMSBr in TFA with thioanisole and p-thiocresol as
scavengers, plus whatever scavengers you need for the other amino acids.
TMSBr takes off Mtr on Arg in an hour at 0 degrees C, so it might be the
best thing for the 9 Pmc groups.
Hope this helps.
Angela C. Murphy
On Fri, 19 May 2000, lisa bibbs wrote:
> Can anyone help. I have a client that needs a 33 mer and then he
> want's 9 Arg's on the end. We had a devil of a time with the 33 mer.
>
> Is there a good strategy for 9R's. I had trouble once with 3R's
> getting the PMC groups off.
>
> Thanks,
> Lisa
> Lisa Bibbs, Ph.D.
> 10550 N. Torrey Pines Rd.
> SP3
> La Jolla, CA 92039
>
> http://corefac.scripps.edu
>
*******************************************
Angela C. Murphy, Chemist
Lab. of Cell Biology, NHLBI, NIH
3 Center Drive, MSC 0301, Rm. B1-22
9000 Rockville Pike
Bethesda, MD 20892-0301 USA
tel.: (301) 496-2324
fax: (301) 402-1519
email: acmurphy@helix.nih.gov
*******************************************
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