Dear Lisa
It might be a bit late to say this but if you use Arg(Pbf) you
will have no problem deprotecting.I have just finished a series of peptides
with 9 non-sequential Arginines and these peptides handled just fine. If you
use Arg(Pmc) then a scavenger mix containing thioanisole is a good start (
see Novabiochems catalogue). Do a couple of small-scale trial cleavages with
different scavenger cocktails and don't be afraid to leave the cleavage
overnight.
Best of luck
Denis Scanlon
>Can anyone help. I have a client that needs a 33 mer and then he
>want's 9 Arg's on the end. We had a devil of a time with the 33 mer.
>
>Is there a good strategy for 9R's. I had trouble once with 3R's
>getting the PMC groups off.
>
>Thanks,
>Lisa
>Lisa Bibbs, Ph.D.
>10550 N. Torrey Pines Rd.
>SP3
>La Jolla, CA 92039
>
>http://corefac.scripps.edu
>
>
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