protein sequencing

From: Anne Stanley (aes7@psu.edu)
Date: Wed May 24 2000 - 15:23:01 EDT


Hi ABRFer's,
I was asked this question I do not know the answer, can somebody help me?

"Our concerns for using PMSF/aprotinin was for protease protection, so they
would only be there in the storage solution. I came across some literature
from Roche/BM that said PMSF has occasionally blocked the amino terminus,
have you seen this as a problem. So, we were thinking of ditching the
PMSF/leupeptin mix for aprotinin instead to protect against proteases. Is
this compound okay? "

Anne

    .-. .-. .-. .-. .-. .-. .-. .-. .-.
 `-' `-' `-' `-' `-' `-' `-' `-' `-'
      Anne Stanley
      Macro Core Facility
      PSU Hershey PA
      Tel (717) 531 6087
      Fax (717) 531 7266
http://www.collmed.psu.edu/core



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