I've used Betaine to eliminate secondary structure of RNA for
reverse transcription. As far as I could find out, there's no
clear notion on how exactly it exerts that effect, so I dont
know if it would help your couse Margarita.
Andreas
At 08:56 AM 5/25/00 -0400, Margarita Almeida wrote:
>Hello. I am having difficulty sequencing GC rich templates. I have a 373
>with XL upgrade and use Big Terminator chemistry. I have heard of a product
>called Betaine. Has anyone used it or does anyone have any other
>suggestions?
>
>Thanks,
>Margarita Almeida
>University of Miami School of Medicine
>Department of Biochemistry and Molecular Biology
>DNA Core Facility
>
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