Hi Margarita,
The DNA Sequencing Research Group (DSRG) has devoted several of its annual
studies to dealing with difficult (GC rich) templates. For a compendium of
approaches using different machines, chemistries, thermocycler conditions etc.
go to the following urls:
http://www.abrf.org/ABRF/ResearchCommittees/dsrg_members.html#Electronic_Pos
ters
http://www.abrf.org/ABRF/ResearchCommittees/dsrcreports/dsrc97.html
http://mbcf.dfci.harvard.edu/abrfdnaseq
Also, the following publication from the DSRG should also be useful:
Adams, P.S., Dolejsi, M.K., Grills, G., McMinimy, D., Morrison, P., Rush, J.,
Goff, S., Milnamow, M., Morgan, A., Karlovitz, M.A., Dial, C.E., and S. Hardin
(1999). An Analysis of Techniques Used to Improve the Accuracy of Automated DNA
Sequencing of a GC-Rich Template. Journal of Biomolecular Techniques 9(4),
9-18. <http://abrf.org/JBT/1998/December98/dec98adams_a.html>.
Ted
At 08:56 AM 5/25/00 -0400, Margarita Almeida wrote:
>Hello. I am having difficulty sequencing GC rich templates. I have a 373
>with XL upgrade and use Big Terminator chemistry. I have heard of a product
>called Betaine. Has anyone used it or does anyone have any other
>suggestions?
>
>Thanks,
>Margarita Almeida
>University of Miami School of Medicine
>Department of Biochemistry and Molecular Biology
>DNA Core Facility
Theodore W. Thannhauser, Ph.D.
Director, BioResource Center
Rm. 149 Biotechnology Building
Cornell University
Ithaca, New York 14853-2703
Phone: (607) 254-4850
FAX: (607) 254-4847
Email: tt34@cornell.edu
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