Esther wrote:
>Does anyone know of a method(reference) for amidating recombinant proteins?
Yes, but the recombinant protein must have the natural
C-terminal Gly extension which becomes oxidized in the 2-step
amidation reaction. Here is our paper for both an expression system
with subsequent amidation:
Expression, processing and secretion of a proteolytically-sensitive
Insect Diuretic Hormone by Saccharomyces cerevisiae, K. S. Copley, S.
M. Alm, D. A. Schooley and W. E. Courchesne, Biochem J., 330,
1333-2340 (1998).
David
-- David A. Schooley Dept. of Biochemistry/330 Univ. of Nevada Reno, NV 89557 schooley@unr.edu tel: (775) 784-4136; fax (775) 784-1419 NOTE NEW AREA CODE: Mandatory after 5/15/99
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