At 04:19 PM 5/30/2000 +0200, Hanno Steen wrote:
>Hi there, is there any protocol available to oxidise the methionine residue
>in peptides in a controlled way so that only the sulfoxide is obtained. I
>tried hydrogenperoxide (3%) and performic acid but in both cases I ended up
>with the sulfonyl compound (the dioxide). Any suggestions are appreciated.
>Thanks in advance, Hanno
>
> __________________
>
> Hanno Steen
>
> Protein Interaction Laboratory (PIL)
> Department of Biochemistry and Molecular Biology
> University of Southern Denmark
> Campusvej 55
> 5230 Odense M
> Denmark
> Phone: +45-65502474 (office), +45-65502470 (lab), +45-65502435 (MS-lab)
> Fax: +45-65933018
> email hanno.steen@cebi.sdu.dk
> URL http//www.pil.sdu.dk
>
Hanno,
Perhaps the following article might be useful:
The Quantitative Oxidation of Methionine to Methionine Sulfoxide by
Peroxynitrite
Daniel Perrin, Willem H. Koppenol
pp. 266-272 ABB 377(2)
Regards,
Dan L. Crimmins
Washington University School of Medicine
Dept. Pathology/Division of Laboratory Medicine
660 S. Euclid Ave., Box 8118
St. Louis, MO 63110
Phone: 314-454-8514; Fax: 314-454-5208
e-mail: crimmins@labmed.wustl.edu
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