I do not know the details,but it matters to get the correct product.I have
seen suggestions,but no definite statements on how to get selective
oxidation,except in Stewart
and Young:Solid Phase Peptide Synthesis.I have the Second edition,and page
27,it states
that:"Methionine can be readily oxidized to the sulfoxide by treatment with
hydrogen peroxide in glacial acetic acid.".And, Bodansky un his Practice of
Peptide Synthesis,also
uses hydrogen peroxide,added in intervals,over 30 min.After sitting for one
hour,absolute
ethanol is added.This is specified for the free amino acid.I have not been
able to find
anything more definite.I hope that it will help.
Hanno Steen <hanno.steen@cebi.sdu.dk>@aecom.yu.edu> on 05/30/2000 09:19:13
AM
Sent by: Association of Biomolecular Resource Facilities
<abrf-request@aecom.yu.edu>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
cc:
Subject: oxidation of methionine
Hi there,
is there any protocol available to oxidise the methionine residue in
peptides in a controlled way so that only the sulfoxide is obtained. I
tried hydrogenperoxide (3%) and performic acid but in both cases I ended up
with the sulfonyl compound (the dioxide). Any suggestions are appreciated.
Thanks in advance,
Hanno
__________________
Hanno Steen
Protein Interaction Laboratory (PIL)
Department of Biochemistry and Molecular Biology
University of Southern Denmark
Campusvej 55
5230 Odense M
Denmark
Phone: +45-65502474 (office), +45-65502470 (lab), +45-65502435 (MS-lab)
Fax: +45-65933018
email hanno.steen@cebi.sdu.dk
URL http//www.pil.sdu.dk
This archive was generated by hypermail 2b29 : Mon Jun 12 2000 - 13:09:42 EDT