Try NaIO4 , 5 equivalent compare to the Met residue, in Imidazole buffer
50 mM, for 5 minutes exactelly. Quench the reaction with 50 eq Met,
Acetic acid and Ethylen Glycol. Purify immidiatly on a Prep HPLC. Be
carefull on the N-Terminal amino acid, if it is Thr or Ser you would
get the coresponding glyoxal peptide. If you have free Cys, they will go
to SOH, SO2H and SO3H.
Good luck
Hanno Steen wrote:
> Hi there,is there any protocol available to oxidise the methionine
> residue in peptides in a controlled way so that only the sulfoxide is
> obtained. I tried hydrogenperoxide (3%) and performic acid but in both
> cases I ended up with the sulfonyl compound (the dioxide). Any
> suggestions are appreciated.Thanks in advance,Hanno
>
> __________________
>
> Hanno Steen
>
> Protein Interaction Laboratory (PIL)
> Department of Biochemistry and Molecular Biology
> University of Southern Denmark
> Campusvej 55
> 5230 Odense M
> Denmark
> Phone: +45-65502474 (office), +45-65502470 (lab), +45-65502435
> (MS-lab)
> Fax: +45-65933018
> email hanno.steen@cebi.sdu.dk
> URL http//www.pil.sdu.dk
-- Matteo Villain Telephone : ++ 41 22 702 5521 Research Assistant E-mail : Villain@cmu.unige.ch DÈpartement de biochimie mÈdicale University of Geneva Rue R.M. Servet, Geneva, CH.
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