Hanno,
If you leave the peptide in solution on the bench for a day or so with
occasional shaking, you obtain a decent amount sulfoxide by
spontaneous oxidation with atmospheric O2 and no sulfonyl compound.
(own old observation).
Anders H. Johnsen
***********************************
Department of Clinical Biochemistry
The National University Hospital
KB3014, Rigshospitalet
Blegdamsvej 9
DK-2100 Copenhagen, DENMARK
phone: (+45) 3545 3007
fax: (+45) 3545 4640
e-mail: johnsen@rh.dk
***********************************
_______________________________ Svaradskiller __________________________________
Emne: oxidation of methionine
Forfatter: Hanno Steen <hanno.steen@cebi.sdu.dk> (Association of Biomolecular
Resource Facilities <abrf-request@aecom.yu.edu>)
Dato: 30-05-00 16:19
Hi there,
is there any protocol available to oxidise the methionine residue in
peptides in a controlled way so that only the sulfoxide is obtained. I
tried hydrogenperoxide (3%) and performic acid but in both cases I ended up
with the sulfonyl compound (the dioxide). Any suggestions are appreciated.
Thanks in advance,
Hanno
__________________
Hanno Steen
Protein Interaction Laboratory (PIL)
Department of Biochemistry and Molecular Biology
University of Southern Denmark
Campusvej 55
5230 Odense M
Denmark
Phone: +45-65502474 (office), +45-65502470 (lab), +45-65502435 (MS-lab)
Fax: +45-65933018
email hanno.steen@cebi.sdu.dk
URL http//www.pil.sdu.dk
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