There are several vendors that offer spin columns in a 96 well format.
Although you would not use all 96 wells, it is more cost effective (in
regards to money and time-sample handling, not to mention reducing
errors
in handling multiple tubes) to purify in the 96 format.
Pre-hydrated
*Princeton Separations
*Edge Biosystems
*Qiagen
*Sigma
and many others
"Home made"
*Millipore
-use a column loader, multiscreen plate, and sephadex G-50
-able to set up only the wells you plan on using, therefore you
may re-use the multiscreen plate for the unused wells.
Precipitation
*AmAc
*NaAc
Magnetic
RapidX (Not certain about the name)
Jennifer Brosius
Sequencing Technologist
ValiGen
300 Pheasant Run
Newtown, PA 18940
Phone:(215) 504-4444
Fax:(215)504-1546
http://www.Valigen.net
-----Original Message-----
From: Erin O'Leary-Jepsen [mailto:oleaerin@isu.edu]
Sent: Monday, June 12, 2000 12:10 PM
To: Recipients of ABRF List
Subject: DNASeq
I currently use an ABI 377-18 DNA Sequencer, and soon we will be
upgrading to a full 377 (36 lanes). What is the best way to purify 36
samples post cycle sequencing? I'm currently using Princeton
Separations Centri-Sep columns, and they work great. Thank you.
Erin
-- Erin O'Leary-Jepsen Idaho State University Molecular Research Core Facility Campus Box 8007 Pocatello, Idaho 83209 Phone: (208) 282-4890 Fax: (208) 282-4570 oleaerin@isu.edu
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