Dear Jaime,
I am sorry for my late reply.
I agree upon your concern regarding the difficulties of selectively
X-linking proteins on cell surfaces without creating a "big mess" in terms
of cell clumps and non-specific protein aggregates.
I think protein X-linking should rather be applied to a much more
restricted system, i.e. a group of proteins suspected to be interacting. I
remember a story from Matthias Mann's lab (CEBI, PIL, Odense, Denmark), in
which they isolated carefully a protein complex by affinity and X-linked
this complex afterwards in order to map it with mass spectrometry
(Rappsilber et al., Anal. Chem. 2000, 72, 267-275).
With regard to X-linkers you'll find an ample selection in the Pierce
Catalogue.
Maybe this helps.
Good luck and best regards,
Martin.
*****************************
Martin Kussmann, PhD
R&D, Protein Analysis /
Mass Spectrometry
CERBIOS-PHARMA S.A.
Via Pian Scairolo 6
CH - 6917 Barbengo (Lugano)
Switzerland
Phone: ++41 - 91 - 985 63 11
Fax: ++41 - 91 - 985 63 25
E-mail: rdbio2@cerbios.ch
-----Original Message-----
From: jaime paba martinez [SMTP:bucaros@unb.br]
Sent: martedi, 23. maggio 2000 21:04
To: rdbio2@cerbios.ch
Subject: RE: protein protein interactions
Dear martin, I have seen a message of you talking about
possibilities to study protein-protein interactions. I
am interested in chemical cross-linking of whole cells
and then PAGE to study protein interaction in a cell
differentiation model, but i Have not found any
reference, could you suggest some? One of my worries is
to make the chemical crosslinking before disrupting the
cell but avoiding the formation of cell clumps.
I would really appreciate your help.
Jaime Paba
University of Brasilia
Brazil
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