The answer is Magnesium-- something like ~6 M magnesium chloride. Tris
buffers are fine.
Susan C. Howard, Ph.D.
-----Original Message-----
From: Roger Murphy [mailto:Roger.Murphy@ludwig.edu.au]
Sent: Tuesday, June 13, 2000 11:33 PM
To: Recipients of ABRF List
Subject: "gentle elution buffer"
A question for the assembled throng....
We're going to use the Pierce "gentle elution buffer" to elute an
acid-sensitive monoclonal antibody from a protein A column. The Pierce
instructions warn of dire precipitation consequences if this buffer ever
comes into contact with phosphate salts...
Does anyone know why? What is the ingredient in their buffer that will
precipitate with phosphates?
Thanks for your anticipated help!
Roger
------------------------------------------------------------
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email Roger.Murphy@Ludwig.edu.au
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