At 2:32 PM +1000 6/14/00, Roger Murphy wrote:
>We're going to use the Pierce "gentle elution buffer" to elute an
>acid-sensitive monoclonal antibody from a protein A column. The
>Pierce instructions warn of dire precipitation consequences if this
>buffer ever comes into contact with phosphate salts...
Both Ca2+ and Mg2+ phosphates will precipitate- water
solubility at neutral pH around 1 mM if I recall correctly.
David
-- David A. Schooley Dept. of Biochemistry/330 Univ. of Nevada Reno, NV 89557 schooley@unr.edu tel: (775) 784-4136; fax (775) 784-1419 NOTE NEW AREA CODE: Mandatory after 5/15/99
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