Many thanks to those who responded (both privately and to the group) to my
question about "gentle elution buffer".
To summarize, the consensus seems to be that it is MgCl2, probably 3.6M at
pH 6.5, with probably ~20mM MES or PIPES to buffer. Also reported to be
compatible with TBS for dialysis/buffer exchange to get rid of the "mystery
buffer".
Thanks again folks - much appreciated.
Roger
------------------------------------------------------------
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email Roger.Murphy@Ludwig.edu.au
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