The unusual cleavages that I got were between glycine and glutamic acid and
between alanine and glutamic acids. I have not seen a cleavage between
between ser and glycine. I also was using ammonium bicarbonate buffer at a
pH of 7.5. I am curious to know what was the enzyme substrate ratio. I
noticed that unusual cleavages by endopeptidases were quite common when the
enzyme concentration was 1:10 or higher.
Amina
Amina S. Woods, Ph.D.
NIDA Intramural Program, NIH
5500 Nathan Schock Drive
Baltimore, MD 21224
Tel: 410-550-1507
Fax: 410-550-2971
e-mail: awoods@intra.nida.nih.gov <mailto:awoods@intra.nida.nih.gov>
-----Original Message-----
From: Association of Biomolecular Resource Facilities
[mailto:abrf-request@aecom.yu.edu]On Behalf Of mike.naldrett
Sent: Monday, June 12, 2000 5:57 AM
To: Recipients of ABRF List
Subject: Digest - anomalous Glu-C cleavage between SER & GLY
Does anybody have experience of endo Glu-C digestions in ammonium
bicarbonate
pH 8.0 cleaving between SER and GLY residues? More completely the sequence
is
YLRSGGKP. Any clues would be welcome.
Thanks
Mike
------------------------------------------------------------
Dr Mike Naldrett - Manager
Protein Sequencing & Proteomics Facility
John Innes Centre(NFL)
Norwich Research Park
Colney
Norwich
NR4 7UH
tel +44-1603-450773 direct line **PLEASE NOTE THE CHANGE OF NUMBERS**
fax +44-1603-450018
e-mail: mike.naldrett@bbsrc.ac.uk
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