Hi Gabriel,
We have reused the Gel Company 96 well porous combs without problem. We
found that if just dried them flat after a run, we could reuse them at
least once without any washing and without any carryover of prior sample.
- George
At 12:36 PM 6/20/00 -0700, Gabriel Archdeacon wrote:
>hi,
> i am going to assume you mean the porous membraine combs made by the
>gel company. to re-use them i usually gently soak them in distilled water.
>you have to be very careful not to bend the teeth, that is the only problem
>with re-using them. you don't need to worry about any remaining samples
>from the last run even if the blue from the loading buffer remains on the
>combs after washing. it is more difficult to re-use the sixty-four lane
>combs and quite difficult to re-use the ninety-six lane combs, but the
>forty-eight lane ones can be used a few times relatively easily.
>
> i would like to thank my friend frank for sharing this knowledge with
>me.
>
>
>
>--
>gabriel archdeacon
>elim biopharmaceuticals
>
>gabriel@elimbiopharm.com
>(650)-827-1984
>fax (650)-827-1986
>
>
>
>you wrote:
>
>> Hello,
>> With reference to the Gel Company combs, I would be most grateful if users
>> could tell me how they 'clean' the combs so that they can be used more than
>> once.
>> Thanks.
>> Martin Whittle
>> Genomic Engenharia Molecular Ltda
>> Rua Itapeva 500/5AB
>> 01332-903 Sao Paulo SP
>> Brasil
>>
>> Tel: +55 11 2881188
>> Fax: +55 11 2884962
>
________________________________________________________________
George Grills
Director
DNA Sequencing, Genotyping, Oligonucleotide, and GeneChip Microarray
Facilities
Albert Einstein College of Medicine
713 Ullmann Building
1300 Morris Park Avenue
Bronx, New York 10461-1602
Tel: (718) 430-2657
Fax: (718) 430-8778
E-mail: grills@aecom.yu.edu
DNA Sequencing: http://leper1.ca.aecom.yu.edu/dnacore
Oligonucleotide: http://sequence.aecom.yu.edu/oligo
________________________________________________________________
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