Hi All!
Does anyone out there have experience with the BioExplore software for the
Finnigan LCQ and it's spectrum deconvolution routine ? I'm new to the
software and have been attempting to get to grips with it by running a few
known samples and have run into a few problems.
I've tried deconvoluting the spectrum for a mixture of known peptides and
the initial deconvoluted spectrum makes no sense, as the masses which I know
the peptides to be do not appear amidst the jumble of artefacts. I've tried
inputting the masses at the "Known Masses" prompt but it makes no difference.
I've also tried accounting for various adducts with no success. Convoluting
back to get the cleaned up spectrum just yields a sequence of peaks at odd
m/z's.
It's particularly annoying as on the unconvoluted spectrum I can see a
range of clear peaks, all of which can be accounted for by the basic charge
states of the component peptides. So why can't the deconvolution process
clean up the spectrum ?
Anyone there help?
Cheers!!
Derek Bradley
Dept. of Medicine
UCL
London
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