Debra
I assume this is a protein? If so, it is likely AEBSF
(aminoethylbenzenesulfonic acid or PefablocÆ). This is often added during
purification to kill any serine protease activity. It can react with Tyr and
a number of other basic residues. Anyway, you can find more information on
the Deltamass home page
http://www.abrf.org/ABRF/ResearchCommittees/deltamass/deltamass.html
or more specifically, for +183 modification, reported by John Stults at
Genentech:
http://www.abrf.org/ABRF/ResearchCommittees/deltamass/AEBSF.html
Good luck.
Mark Hail
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Novatia Corporation
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Yardley, PA 19067
215-321-3130 (office)
267-981-5914 (mobile)
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hailm@enovatia.com
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-----Original Message-----
From: Association of Biomolecular Resource Facilities
[mailto:abrf-request@aecom.yu.edu]On Behalf Of Debra A Burdick
Sent: Thursday, June 22, 2000 2:44 PM
To: Recipients of ABRF List
Subject: MS
Recently I have seen an adduct peak in my samples run on an LC/MS - an H.P
(Agilent) MSD single quadrapole. The samples have an extra 183 or 2x (366)
mass units. Different source samples, different columns, and new buffers
were tries and I still see the same adduct. Is this anything that has been
seen before?
Dr Debra Burdick
Dupont Pharmaceuticals
302-695-3397
Debra.A.Burdick@dupontpharma.com
This archive was generated by hypermail 2b29 : Fri Jun 30 2000 - 07:51:55 EDT