I have had some annoying/bad experiences with protein that may contain
transition mmetal cations! It is not clear to me whether these/such cations
may "poison" Edman degradation. Best of luck.
alex
Alexander W. Bell
Anatomy and Cell Biol
McGill University
----- Original Message -----
From: Sandy Kielland <uunet!uvic.ca!Kielland@mail.uu.net>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Sent: Tuesday, June 27, 2000 12:54 PM
> Hello all;
> Does anyone know if the use of Ni-NTA columns would interfere with the
> ability to perform N-terminal analysis? The samples were on PVDF. I've run
> quite a few "blocked" samples lately that shouldn't have been blocked.
>
> Regards,
>
> Sandy Kielland
> Victoria Protein Microchemistry Centre
> Department of Biochemistry and Microbiology
> University of Victoria
> Victoria B.C. CANADA
> phone: (250) 7218884
> FAX (250) 7218855
> Email Kielland@UVic.ca
> Webpage http://www.proteincentre.com
>
>
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