You do not find anhydrotrypsin? Don't worry!
A simple ion-exchanger like MONO-S run in 0.1% TFA at pH 2.5 will do the
same job because the C-terminal peptide will not bind (unless it ends with
R or K.). FEBS LETT.273,168-172 (1990); (but read Mono-S instead of Mono-Q
on the first page, Materials and methods, 3rd para ). I have used this
procedure repeatedly with a lot of proteins and it always worked except
with the basement membrane protein nidogen-1 which ends with ..RK.
Best regards
K.Mann
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