HPLC

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IĄd like to use three TSK-Gel SW ( silica-based ) hplc gel filtration
columns for protein purification. Anyway, i have been told that these
columns have been used with detergent ( i don`t have any information
what kind of ... ). So, what`s best to remove any detergent from the
columns: gradients of acetonitrile in water or the
chloroform:MeOH:water (2:5:2) mixture described in an earlier posting (
might it damage the column ? ) or something else ?
Second: Is there any easy method ( physical , biotest, spectroscopical )
to detect trace impurities of detergent in the effluent ?

Any hints are welcome !!!

Thanks, Timm

T Maier
FU Berlin
Macromolecular Crystallography
tmaier@chemie.fu-berlin.de

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