I have a client who is having difficulty getting sequences out of his
samples on my 373XL. He's using Lambda-phage DNA which is approximately 40
Kb in size. His vector is lambda-phage, and he is trying to sequence the
insert. He first tried an internal primer, but saw no sequence, then tried
a universal primer, and still there was no sequence. He's been
precipitating with ethanol. Because of the large size, instead of
submitting the normal .5µg amount, he's been submitting 1.0 - 2.0 µg.
Should he be submitting smaller quantities, such as .5µg? If anyone can
help with some possible solutions, I would greatly appreciate it.
___________________________________________________________________
Susan K Fetics
Laboratory of MicroChemistry
Lindsley F. Kimball Research Institute
The New York Blood Center
310 E. 67th St., 3rd floor
New York, NY 10021
phone: (212) 570-3188
sfetics@nybc.org
For information about the MicroChemistry lab at NYBC or for request forms,
please visit:
http://www.nybloodcenter.org/framesets/FS-3C17.htm
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