denaturing agarose gels

From: Winnell H. Newman (winnell@ncsu.edu)
Date: Tue Jul 11 2000 - 14:49:36 EDT

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Hi all:

Someone asked me about protocols for making denaturing agarose
gels...any hints or writeups would be appreciated.

I have protocols for 8M urea 20% polyacrylamide gels...but we can't seem
to make the transition to agarose. We've dropped urea to 5M and varied
the mixing pattern (urea in TAE, then add agarose; agarose in TAE then
add urea).

thanks

Winnell

-- Winnell H. Newman, Director, Nucleic Acids Facility (NAF) North Carolina State University, Department of Biochemistry 128 Polk Hall, Box 7622 Raleigh, NC 27695-7622

Nucleic Acids Facility Web Page http://www.ncsu.edu/naf Email = mailto:winnell@ncsu.edu VOX (919) 515-3463 FAX (919) 515-2047

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