Hello everyone,
A co-worker of mine has this problem:
His current project is to synthesize 15-20 peptides that span the length
of an interesting protein. Then he will use these peptides in a binding
assay to determine the "binding site."
As Murphy's law would have it, the synthesis of the peptide which is his
bet for "Most likely to contain the binding site", has given multiple
N-termini when sequenced. I suspect (from the sequence) that it is
something like 50% A, 25% B with several smaller peptides making up the
last 25%.
We have no HPLC in our lab (I have been telling my boss ever since I
started that we need one -- but of course we don't have the money).
Is there anyone out there who could take this peptide (it is a 22mer,
and we have ~35mg) and purify it for us ?
Thank you,
-- Rebecca P. Ettling
Biotechnology Resource Laboratory
Protein Sequencing and Peptide Synthesis Facility
Medical University of South Carolina
173 Ashley Avenue, Room 733D BSB
Charleston, SC 29403
Tel.: (843) 792-1271
Fax: (843) 792-1264
If I have seen farther than others, it is because I was standing on the
shoulders of giants.--Isaac Newton
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