Re: 2D and protein measuring

From: Katheryn Resing (Katheryn.Resing@Colorado.EDU)
Date: Thu Jul 20 2000 - 05:06:43 EDT

Next message: jaime paba martinez : "protein measuring"
Previous message: LANGE, GARY W [PHR/1005]: "RE: Recommendations on MALDI instruments"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

When we have a total cell extract in an SDS containing buffer, we acetone
precipitate, resuspend in 10% formic (or whatever denaturing buffer is
compatable with your protein assay) and then measure the protein.

Katheryn Resing

Next message: jaime paba martinez : "protein measuring"
Previous message: LANGE, GARY W [PHR/1005]: "RE: Recommendations on MALDI instruments"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

This archive was generated by hypermail 2b29 : Fri Aug 18 2000 - 07:56:58 EDT