Scott - thank you for the comprehensive summary; I wish we would do this
more often. Sometimes we don't know if ANY responses were received, or if
MANY were (but just not posted). We posted a query a few weeks ago on
experiences with Agilent's new MS Ion Trap, and got only one response from a
vendor. While it was nice to get it from them, I didn't feel it needed to
be posted back to the group. I often see other such questions without ever
seeing if info was exchanged. It is a loss to all of us when this happens.
BTW: In terms of N-terminal sequencing, our lab offers this service as
well, but only under GLP/cGMP conditions. It didn't appear as though this
level of compliance was necessary from your question.
Nadine Ritter, Ph.D.
Director of Analytical Services
BioReliance
nritter@bioreliance.com
> -----Original Message-----
> From: Leigh, Scott [SMTP:SLeigh@cson.com]
> Sent: Wednesday, July 26, 2000 12:47 PM
> To: Recipients of ABRF List
> Subject: protein sequencing facilities
>
> I recently asked for suggestions for places that would perform N-terminal
> sequencing on tryptic peptides:
>
> I've got a friend in the Boston area who is looking for a lab to sequence
> some peptides from a tryptic digest. Can anyone recommend a lab to perform
>
> N-terminal sequencing and maybe provide any price information?
>
> As would be expected there were a large number of responses. Thanks to
> everyone who replied. Since only one of the responses was a reply to the
> discussion group, all the rest were direct replies to me, I've listed all
> of
> them below in case someone else would like similar information.
>
> There were 23 responses:
>
> 1)
> Midwest Analytical does excellent N-terminal sequencing. Contact
> Dave McCourt at 314-487-8622 for information about prices.
>
> 2)
> It can be done at the Biotech. Resource Center at Cornell.
> http://brcweb.bio.cornell.edu/
> for price info/sample submission.
>
> 3)
> Plase go to our webside
> www.biotech.ubc.ca/naps/napshome. htm
>
> 4)
> Here at the NAPS Unit of the Biotechnology Lab, at the University of
> British Columbia, we do N-terminal sequencing of proteins and peptides
> (along with many other services). One can visit our website (see the
> address below), email or call me, for additional info.
> http:/www.biotech.ubc.ca/services/naps/
>
>
> 5)
> You can try Bill Lane's sequencing group at Harvard:
> http://www.golgi.harvard.edu/Microchem/index.html
>
>
> 6)
> A friend here in St. Louis, who is on the abrf email group, called with
> your
> address. Said you were interested in having some peptides sequenced.
> Please give me a call if you are interested or have any questions. I'd be
> happy to help out. Thanks.
>
> Regards,
> Dave
>
> David W. McCourt
> Midwest Analytical, Inc.
> 11141E South Towne Square
> St. Louis, MO 63123
>
> phone: 800-481-0790 / 314-487-8622
> email: mccourt@mastl.com
> http://www.mastl.com
>
>
> 7)
> Sequencing labs are only as far away as the FedEx box. I use Argo
> BioAnalytica in New Jersey. Contact StvTindall@AOL.com or 973-605-2100.
> Recent runs included tryptic digest of 2-3 pmol of a 75k Da band;
> sequencing
>
> identified one protein and gave 6 peptides from the other.
>
>
> 8)
> our protein chemistry and mass spec facility at the University of Napoli
> is
> routinely carrying out protein and peptides sequencing for customers. You
> can give a look to our web site at
> http://sco.technapoli.it/massa/Home.html.
> Prices are depending upon the number of analysis and can be discussed by
> mail if you are interested.
>
> 9)
> We are kind of close to Boston. I work at Cornell in Ithaca NY and
> we have a core facility. I regularly do tryptic digests and the
> corresponding peptide maps and N-terminal sequencing, plus we just got a
> top of the line MALDI-TOF MS and an electrospray ion trap MS, so we are
> pretty well outfitted for protein analysis. I also think our rates are
> good, comparatively.
> Your friend can check out our web-site if he/she wants at:
>
> http://brcweb.bio.cornell.edu/
>
>
> 10)
> We offer Edman sequencing services to outside investigators as
> well as micro RP-HPLC separations of tryptic digests. Check out
> our website for more details and pricing info.
>
> pdtc.rockefeller.edu
>
> Yale University and Harvard also offer similar services and are
> geographically closer to Boston. Here are links to their sites as well.
>
> http://info.med.yale.edu/wmkeck/
>
> http://golgi.harvard.edu/microchem/
>
>
> 11)
> We use Mike Berne's lab in Tufts University (Medical school). He has very
> good service and the best price.
> His phone # 617-636-2422 e mail: mberne@opal.tufts.edu
>
>
> 12)
> we rutinely sequence proteins & peptides by means of Edman sequencer or
> MS-based techniques for pretty good price. But I am not sure if your
> friend
> wants to send his samples to Europe.
>
> Yours,
>
> Jan Sklenar
> >Institute of Microbiology
> >Videnska 1083
> >142 20 Prague
> >Czech Republic
> >tel. 00420-2-4752383
>
>
> 13)
> We provide amino terminal sequencing on a PE Biosystems Procise
> 494. We run a 2 pmol bovine beta-Lactoglobulin standard. We also provide
>
> homology
> searching if desired at no extra charge. Our fee is $40 per amino acid
> with a 6 cycle minimum charge whether or not the sample shows a sequence
> (assuming
> that not isn't the result of instrument/human error). Most tryptic
> peptides are 8-15 amino acids long if you want to estimate cost. As an
> aside, we also do
> protein identification by mass spec analysis, both MALDI-TOF and
> nano-capillary ESMS, of tryptic digests. My contact info is below if you
> are interested.
> Regards, Mark.
>
>
> Mark W. Crankshaw
> Protein and Nucleic Acid Chemistry Laboratories
> Department of Molecular Biology and Pharmacology
> Washington University School of Medicine
> 660 South Euclid Avenue, Campus Box 8103
> St. Louis, MO 63110 USA
>
> voice: (314) 362-0285
> FAX: (314) 362-4698
> email: crank@pcg.wustl.edu
> web: http://pharmdec.wustl.edu/pnacl.home.html
>
>
> 14)
> My laboratory can provide Edman protein sequencing facility to your
> friend. Our charges are $200 for the first five residues and then $25 per
> cycle. The rurn around time is fast.
> Thanks
>
> Satya Yadav, Ph.D.
> Director, Molecular Biotechnology Core
> The Lerner Research Institute
> Cleveland Cllinic Foundation
> 9500 Euclid Avenue
> Cleveland, OH 44195
>
> Tel: (216) 445-7095
> Fax: (216) 444-2998
> Email: yadavs@ccf.org
>
>
> 15)
> The MSSSF at Michigan State University accpts ouside samples. We are
> equipped with both high through-put and high sensitivity sequencers. Our
> turn around time is 4 to 5 days.
>
>
> Joe Leykam
>
> Joseph F. Leykam Phone (517)353-4824
> Director. MSSSF FAX (517)353-9334
> Dept. of Biochemistry RM 223
> Michigan State University E-mail msssf@msu.edu
> East Lansing MI 48824
>
>
> 16)
> Try Dick Cook at MIT 617-253-1685 or Bill Lane at Harvard 617-495-4043.
>
>
> 17)
> We can do that sort of work here at Macromolecular Resources. I'm
> attaching
> our web link. http://mmr.bmb.colostate.edu
>
>
> 18)
> I don't know if you're looking for someone local to do the sequence
> analysis of the peptides, but we offer service to off campus customers.
> If
> the individual is from an academic setting, the rates are;
>
> $85 for 6 amino acids
> $150 for up to 25 amino acids
> $250 for more then 25
>
> If they are from industry, the rates are higher...
>
> $300 for 6 amino acids
> $450 for up to 25 amino acids
> $700 for more then 25
>
> Let me know if they are interested in our services. We have an Applied
> Biosystems Procise sequencer and or lab has been offering the service for
> 12 years now.
>
> Thanks!
>
> Mary
>
> Mary Bower
> Instrument Specialist
> Purdue University
> Biochemistry Dept.
> W. Lafayette, IN 47907
>
> Ph. (765)494-6540
> Fax (765)494-7897
> email; Bowerma@purdue.edu
>
>
> 19)
> There are a lot of us out here. You can get my pricing and application
> sheet at our website listed below. We are very competitive, very reliable
> and best of all fast with the results.
>
> Eric
> --
> Eric F. Howard
> eric.f.howard@vanderbilt.edu
> 614 Light Hall
> Department of Biochemistry
> Vanderbilt University
> Nashville, TN 37232
>
> http://www.mc.vanderbilt.edu/cancer/intranet/resource9.html
>
>
> 20)
> We do N-terminal protein sequencing with ABI 477. Our pricing is $60 for
> set up and $15 per amino acid. Our turn around time is within three days.
> If interested, please contact me. Thanks.
>
> Bob Lee, Ph.D.
> E-102 MSB, m/c 937
> 835 S. Wolcott Ave.
> Chicago, IL 60612
> Tel: (312)996-1411
> Fax: (312) 996-1898
>
>
> 21)
> Try Michael Bernes at Tufts University 617-636-6220, inthe past they have
> sequenced samples,
> I don't remember the exat cost, but I do remember it was reasonable.
>
>
> 22)
> We would be happy to do it .Please see our web page at
> www2.utmb.edu/proch/
> . Our charge is $15 per residue/cycle with no set up fee cost now .
>
> Alex Kurosky , PhD
> Director , UTMB Protein Chemistry Laboratory
> Phone : 409-772-2771
>
>
> 23)
> M-Scan is a well respected lab in Philadelphia that does both N-terminal
> sequencing
> and mass spectrometry. I have audited them in a quality inspection and
> they
> are very
> good. http://www.m-scan.com/
This archive was generated by hypermail 2b29 : Fri Aug 18 2000 - 07:56:58 EDT