Re: Racemization/polarimeters

From: I.L. Rodionov (rodionov@fibkh.serpukhov.su)
Date: Thu Jul 27 2000 - 16:36:10 EDT


Dear Jim:

Racemization in case of peptide, composed of at least 2 hiral amino
acids, will produce diastereomeric peptide which should resolve either
under standard HPLC conditions, or using HR ion exchange
chromatography. (I recommend to try any AA analyzer with post-column
ninhydrine without modification of the program). Quantitation is
straightforward.

Good luck!

Igor

------------------------------------------------

Igor Rodionov
Laboratory of Peptide Chemistry
Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
6 Science Avenue, Pushchino, Moscow Region
142292, Russian Federation
rodionov@fibkh.serpukhov.su



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