Deb
Well this does seem possible although the issues you bring up are quite true. I have
listened to some heated debates on the validity of the data by those much more in touch
with this area than I. Dick Smith's lab is one of the groups working in this area.
Maintaining physiological pH is one issue that is addressed usually by running in 10mM
NH4OAc, I think.
The source conditions for ESI have to be REALLY soft it seems. Lower temps and gas
settings than usually used. I'd want to have additional 'control' species that do not
bind included in my exp. set as well.
I have struggled with the issue of quoting the bibliography section I included at the end
(copyright-wise). If you want to yell at me go ahead, I will listen. As I state, it is
not my work and can be found and downloaded on the web as this posting will eventually be.
Matt Sweeney
mattsweeney@earthlink.net
Mass Spec Consulting
Training/Operations/Consulting/Method Development
LC/MS Pharmacokinetics, Peptides, Proteins, Metabolism,
Maintenance Classes, Specialist in Finnigan Equipment and Software
I found some papers at the ASMS/elsevier site where many of the JAMS papers are posted
(fast-track articles they call it).
http://www.elsevier.com/homepage/saa/webjam/access/fast-toc.shtml
the ones that seem to apply are
Preservation and Detection of Specific Antibody-Peptide Complexes
by Matrix-assisted Laser Desorption Ionization Mass Spectrometry
Janna G. Kiselar and Kevin M. Downard
http://www.elsevier.com/homepage/saa/webjam/access/letters/jam1593.pdf
Non-covalent associations of glutathione S-transferase and ligands: a
study using electrospray quadrupole/time-of-flight mass spectrometry
Masaki Ishigai, James I. Langridge, Robert S. Bordoli, and Simon J. Gaskell
http://www.elsevier.com/homepage/saa/webjam/access/letters/jam1504.pdf
Gas-Phase Stability of Double-Stranded Oligodeoxynucleotides and
Their Noncovalent Complexes with DNA-binding Drugs is Revealed by
Collisional Activation in an Ion Trap
Katty X. Wan 1 , Toshimichi Shibue 2 and Michael L. Gross
http://www.elsevier.com/homepage/saa/webjam/access/letters/jam1537.pdf
and for easy/quick use here is a section of the bibliography from Ishigai et al. (it looks
really useful) with a couple from the Wan et al paper thrown in for good measure,
(1) Smith, R. D.; Bruce, J. E.; Wu, Q.; Lei, Q. P. New mass spectrometric methods for the
study of noncovalent associations of biopolymers Chem. Soc. Rev. 1997, 26, 191-202.
(2) Smith, R. D.; Light-Wahl, K. J.; Winger, B. E.; Loo, J. A. Preservation of
non-covalent
associations in electrospray ionization mass spectrometry: multiply charged polypeptide
and protein dimers Org. Mass Spectrom. 1992, 27, 811-21.
1. Smith, R. D.; Light-Wahl, K. J. The observation of non-covalent interactions in
solution by electrospray ionization mass spectrometry: Promise, pitfalls and
prognosis. Biol. Mass Spectrom. 1993, 22, 493-501.
2. Smith, D. L.; Zhang, Z. Probing noncovalent structural features of proteins by mass
spectrometry. Mass Spectrom. Rev. 1994, 13, 411-429.
3. Przybylski, M.; Glocker, M. O. Electrospray mass spectrometry of
biomacromolecular complexes with non-covalent interactions - new analyticalperspectives
for supramolecular chemistry and molecular recognition processes.
Angew. Chem. Int. Ed. Engl. 1996, 35, 806-826.
4. Smith, R. D.; Bruce, J. E.; Wu, Q. Y.; Lei, Q. P. New mass spectrometric methods for
the study of noncovalent associations of biopolymers. Chem. Soc. Rev. 1997, 26, 191-
202.
5. Loo, J. A. Studying non-covalent complexes by electrospray ionization mass
spectrometry. Mass Spec. Reviews 1997, 16, 1-23.
6. Suckau, D.; Shi, Y.; Beu, S. C.; Senko, M. W.; Quinn, J. P.; Wampler, F. M., III;
McLafferty, F. W. Coexisting stable conformations of gaseous protein ions. Proc.
Natl. Acad. Sci. USA 1993, 90, 790-793.
7. Feng, R.; Konishi, Y. Stepwise refolding of acid-denatured myoglobin: evidence from
electrospray mass spectrometry. J. Am. Soc. Mass Spectrom. 1993, 4, 638-645.
8. Benjamin, D. R.; Robinson, C. V.; Hendrick, J. P.; Hartl, F. U.; Dobson, C. M. Mass
spectrometry of ribosomes and ribosomal subunits. Proc. Natl. Acad. Sci. U. S. A
1998, 95, 7391-7395.
9. Fitzgerald, M. C.; Chernushevich, I.; Standing, K. G.; Whitman, C. P.; Kent, S. B. H.
Probing the oligomeric structure of an enzyme by electrospray ionization time-of-flight
mass spectrometry. Proceedings of the National Academy of Sciences of the United
States of America 1996, 93, 6851-6856.
10. Loo, J. A.; Ogorzalek Loo, R. R.; Andrews, P. C. Primary to quaternary protein
structure determination with electrospray ionization and magnetic sector mass
spectrometry. Org. Mass Spectrom. 1993, 28, 1640-1649.
11. Murayama, K.; Taka, H.; Kaga, N.; Fujimura, T.; Mineki, R.; Shindo, N.; Morita, M.;
Hosono, M.; Nitta, K. The structure of Silurus asotus (catfish) roe lectin
Identification of a noncovalent trimer by mass spectrometry and analytical
ultracentrifugation. Anal. Biochem. 1997, 247, 319-326.
12. Rostom, A. A.; Robinson, C. V. Disassembly of intact multiprotein complexes in the
gas phase. Curr. Opin. Struct. Biol. 1999, 9, 135-141.
13. Vis, H.; Dobson, C. M.; Robinson, C. V. Selective association of protein molecules
followed by mass spectrometry. Protein Sci. 1999, 8, 1368-1370.
14. Ganem, B.; Li, Y.-T.; Henion, J. D. Observation of noncovalent enzyme-substrate and
enzyme-product complexes by ion-spray mass spectrometry. J. Am. Chem. Soc. 1991,
113, 7818-7819.
15. Feng, R.; Castelhano, A. L.; Billedeau, R.; Yuan, Z. Study of noncovalent enzyme-
inhibitor complexes and metal binding stoichiometry of matrilysin by electrospray
ionization mass spectrometry. J. Am. Soc. Mass Spectrom. 1995, 6, 1105-1111.
16. Ganem, B.; Li, Y.-T.; Henion, J. D. Detection of noncovalent receptor-ligand
complexes by mass spectrometry. J. Am. Chem. Soc. 1991, 113, 6294-6296.
-----Original Message-----
From: Deb McMillen <mcmillen@morel.uoregon.edu>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Date: Friday, July 28, 2000 1:36 PM
Subject: mass spec
>Hi, all,
>
>Is it possible to definitely say that two molecules, a protein and a
>peptide, associate non-covalently using MALDI or electrospray........
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