Yes, the anhydrous forms from other suppliers should work in your 433,
they work in my 430. Just remember to make up your HBTU/HOBt in DMF, not
NMP. You can get some very pure DMF over molecular sieves from Fluka.
I make up my own HBTU/HOBt when I have just one or a few small peptides to
make and don't want to "waste" a whole 100 mmole bottle.
Hope this helps.
Angela C. Murphy
On 28 Jul 2000, Jim Lee wrote:
> ABRF,
> Does anyone have an alternate supplier for HOBT or HBTU? Applied Bio(welcome back to the old name) has been our supplier but since June of this year we have been told that these reagents have been backordered. We have been told 4 different dates of when ABI will be shipping these reagents. Is there anyone out there who has a supply of these reagents who could spare a bottle HOBT or HBTU? Is the non-anhydrous reagent that is available from other suppliers an alternative that will work on the ABI433 synthesizer? Thanks in advance,
>
> Jim Lee
> Molecular Biology Core Facilities
> Dana Farber Cancer Institute
> 44 Binney Street
> Boston, MA 02115
> 617-632-3082
> j_lee@dfci.harvard.edu
>
*******************************************
Angela C. Murphy, Chemist
Lab. of Cell Biology, NHLBI, NIH
3 Center Drive, MSC 0301, Rm. B1-22
9000 Rockville Pike
Bethesda, MD 20892-0301 USA
tel.: (301) 496-2324
fax: (301) 402-1519
email: acmurphy@helix.nih.gov
*******************************************
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