Lys C from Achromobacter lyticus is the only one that I would use. I think
a lot of other people also didn't have good results with the other type. As
far as I know the only source for Achromobacter Lys-C is Wako. Does anyone
know of another source of Achromobacter Lys-C??
-----Original Message-----
From: Marcelo Valle de Sousa [mailto:mvsousa@unb.br]
Sent: Wednesday, August 02, 2000 11:05 AM
To: Recipients of ABRF List
Subject: Digest: Lys-C and Arg-C
We intend to digest proteins in gel by using Lys-C and Arg-C
enzymes. We then have the following questions:
1) Do these enzymes work well for protein identificatioin by peptide
mass fingerprinting?
2) Are there any special protocols for Lys-C from Lysobacter
enzymogenes (Sigma P3428) and Arg-C from mouse submaxillary gland (Sigma
P 6056) or they work equally well when obtained from different sources
and companies?
3) Could the gel be silver stained or should it be coomassie blue
stained?
4) Could anyone send specific protocols for protein in gel digestion
with the above Sigma enzymes?
Thank you very much in advance.
------------------------------------------------------
Prof. Marcelo Valle de Sousa
Director
Brazilian Center for Protein Research and Services
Laboratory of Protein Chemistry and Biochemistry
Departament of Cell Biology
University of Brasilia
Brasilia - DF Brazil
CEP 70910-900
Tel: +5561-307-2142
Fax: +5561-272-4548
e-mail: mvsousa@unb.br
http://www.lbqp.unb.br/cbsp/
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