Dear Wei
a solution we developed for screening for cysteine-rich peptides in complex
mixtures is the combination of carboxyamidomethylation followed by
differential MALDI-TOF-MS. We are currently preparing a manuscipt and
meanwhile, a poster will be presented at the MPSA in Charlottesville,
Virginia.
Through application of this method on body fluids we have identified many
peptides rich in disulphide bonds.
Best regards
Michael Schrader
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> -----Urspržngliche Nachricht-----
> Von: Wei Wu [SMTP:wuwei1@cem.msu.edu]
> Gesendet am: Sonntag, 30. Juli 2000 02:19
> An: Recipients of ABRF List
> Betreff: S-S peptides
>
> Hi,
>
> Our lab is gonna do some research on peptides linked by disulfide. Like
> two peptides linked by one disulfide or three peptides linked by two. One
> possible source is from digestion of native/unreduced proteins that has
> disulfide bonds. The question is about detection of those special species
> out of tons of digestion fragments. Is there some special absorption
> characeristics that can be used? Or any other means?
>
> Thanks for your attention.
>
> Wei Wu
> Department of Chemistry
> Michigan State University
> East Lansing, MI 48823
> 1-517-353-6767
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