Here is last week's request:
I have received an inquiry from a researcher who wants to quantitate
glutamic acid and glutamine in physiological samples. I would like to refer
her to someone in the USA who performs this service. Please let me know if
you can help, and I'll compile and post the replies.
Thanks.
And here are the replies I received:
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From: "wac@u.arizona.edu" <wac@u.arizona.edu>
I run physio AAA here at the U. of Arizona. If you are interested
please e-mail me back (direct). Hope to hear from you.
Wally (Wallace Clark)
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From: Bob Lee <boblee@uic.edu>
We do this all the time with fast turn around and low cost. If interested,
please contact me. Thanks.
Bob Lee, Ph.D.
Director of Protein Research Lab. at UIC
E-102 MSB, m/c 937
835 S. Wolcott Ave.
Chicago, IL 60612
Tel: (312) 996-1411
Fax: (312) 996-1898
e-mail:boblee@uic.edu
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From: fperini@unmc.edu
I can do these types of analyses, using AccQTag chemistry. Glutamic elutes
before glycine, while Glutamine elutes right after it, in this system.
The neat thing about AccQTag is that it is not affected by many impurities
which give problems with other chemistries. Just keep ammonium ion and Tris
rather low as they compete for the reagent.
Fulvio Perini,Ph.D.
986805 Nebraska Med.Cntr.
Omaha,NE 68198-6805
ph:402-559-6184/FAX 402-559-4651
fperini@unmc.edu
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From: Dana Cass <dmcass@ucdavis.edu>
The quantitation of glu and gln in physiological samples is something that
we are able to do. The cost for academic customers is $40.00 per sample.
Non-academics are charged $60.00 per sample. Please visit our website,
http://psl.ucdavis.edu, to find out the specifics of acceptable
samples. If you have any questions, please call me at 530-752-6228.
The machine that is capable of analyzing the physiological samples is very
popular and is being used half time for long-term projects. As a result, I
have no way of estimating the turn around time for data. Usually, if the
requestor has only a few samples, we are able to run them and return data
in 1-2 weeks. Large amounts of samples will take longer.
Please let me know if you have any questions.
Sincerely,
Dana Cass
Molecular Structure Facility
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Thank you to all who replied.
Carol
Carol Manley Beach, Ph.D.
Protein Sequence Analysis
Macromolecular Structure Analysis Facility
232 Combs Cancer Research Building
University of Kentucky
Lexington, KY 40536-0096
cmbeach@pop.uky.edu
NOTE NEW AREA CODE: Mandatory after Oct 1, 2000
(859)257-4932 (office)
(859)257-4475 (lab)
(859)323-3909 (FAX)
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