Hi everyone,
I have a colleague who has a separation problem -
here she is:-
Hi
I am trying to separate 2 forms of trimeric type I collagen. (mw 330 kD)
There are two different forms that differ by about 60kD that I am trying
to separate. My big problem is that I need to keep them in their
trimeric form and so can't reduce them.
I have tried a 5% SDS PAGE gel and managed to get the protein into the
gel - but no bands - just a big old smear! What else can I try?? Would
an agarose based gel be better and if so what kind of %???
I'd be grateful for any advixce for working with these large proteins
Thanks in advance................Tracy Stewart
Ok - I hope that someone out there can help - thanks in advance.
Best wishes from Scotland,
Bryan.
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