Hi, as to your questions about the possibility of reaction between TBP and
iodoacetamide, I think they do react with each other. I once did a small
experiment with TCEP (a TBP equivalent) and iodoacetamide, using P31-NMR. It
turned out that the two react very fast.
----- Original Message -----
From: Guo-An Wang <gawang@MIT.EDU>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Sent: Monday, August 07, 2000 9:10 PM
Subject: digest
> Dear ALL:
>
> What I am thinking to do is something like this. The protein is alkylated
> with iodoacetamide-like and cut with CNBr before subjected to 2D
> electrophoresis. I am not keen on such protein chemistry, wondering these
> questions: Can I use TBP (tributyl phosphine) instead DTT for the
reduction
> step? Does TBP react with iodoacetamide? Do I have to get rid of these
> reagents before doing CNBr cleavage? Does CNBr cleave at the newly formed
> thioether site (cysteine)? I know CNBr clavage is quite specific, but I
> havenot seen any 2D pictures of CNBr peptides. Can anybody tell me how
they
> perform?
> Any responses to these questions are highly appreciated!
> Thank you!
> Guo-An
>
>
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