Does anyone use membrane combs from the Gel Company? I have tried their
ficoll method of loading the comb and find that auto-tracking doesn't work
so well. I am using Genescan for the ABI 377 and 96 well upgrade. Has
anyone been able to auto-track a gel? If so, can you tell me what method
works best? I do appreciate any help you can offer.
Karen Clare
RiceTec, Inc.
281-393-3502
-----Original Message-----
From: Wei Wu [mailto:wuwei1@pilot.msu.edu]
Sent: Tuesday, August 08, 2000 7:51 AM
To: Recipients of ABRF List
Subject: Re: digest
Hi, as to your questions about the possibility of reaction
between TBP and
iodoacetamide, I think they do react with each other. I once
did a small
experiment with TCEP (a TBP equivalent) and iodoacetamide,
using P31-NMR. It
turned out that the two react very fast.
----- Original Message -----
From: Guo-An Wang <gawang@MIT.EDU>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Sent: Monday, August 07, 2000 9:10 PM
Subject: digest
> Dear ALL:
>
> What I am thinking to do is something like this. The
protein is alkylated
> with iodoacetamide-like and cut with CNBr before subjected
to 2D
> electrophoresis. I am not keen on such protein chemistry,
wondering these
> questions: Can I use TBP (tributyl phosphine) instead DTT
for the
reduction
> step? Does TBP react with iodoacetamide? Do I have to get
rid of these
> reagents before doing CNBr cleavage? Does CNBr cleave at
the newly formed
> thioether site (cysteine)? I know CNBr clavage is quite
specific, but I
> havenot seen any 2D pictures of CNBr peptides. Can anybody
tell me how
they
> perform?
> Any responses to these questions are highly appreciated!
> Thank you!
> Guo-An
>
>
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