Hi Karen:
I'm using the 64 lane membrane combs (ficoll method) from the Gel Company for
sequencing, and I also include 1.5% of their lane tracker DET50 in my sample
dye. This produces a green spot strong enough to be picked up by the tracker,
but weak enough that it doesn't interfere with the other peaks. I have near
perfect tracking.
Hope this helps!
Sheryl Christofferson
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Oklahoma Medical Research Foundation
DNA Sequencing Facility
Oklahoma City, Oklahoma
(405) 271-7393 ext. 34565
www.omrf.ouhsc.edu/OMRF/Core/DNASEQ/
sherylc@omrf.ouhsc.edu
"Karen M. Clare" wrote:
> Does anyone use membrane combs from the Gel Company? I have tried their
> ficoll method of loading the comb and find that auto-tracking doesn't work
> so well. I am using Genescan for the ABI 377 and 96 well upgrade. Has
> anyone been able to auto-track a gel? If so, can you tell me what method
> works best? I do appreciate any help you can offer.
> Karen Clare
> RiceTec, Inc.
> 281-393-3502
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