Brett,
The ion mapping software for determining phosphorylation sites can be used
for static nano spray, infusion or chromatographic data. The view was
designed for an infusion experiment where the MS/MS precursor is incremented
in an ion mapping experiment. It can also be used for chromatographic MS/MS
data but the trace is collapsed to a parent vs product ion view and
retention data is not displayed, but you can still see which parents lose
the characteristic m/z 49.
There is also a feature in Xcalibur 1.2 which is a neutral fragment filter
which will display specific neutral losses from chromatographic MS/MS runs,
this can be accessed in the qual browser range view. An example of this can
be seen on the Thermoquest Web site under the products and download pdf
section.
Iain Mylchreest
Finnigan
San Jose
> -----Original Message-----
> From: Brett Phinney [SMTP:phinney1@msu.edu]
> Sent: Friday, August 11, 2000 6:50 AM
> To: Recipients of ABRF List
> Subject: LCQ Deca ion Mapping
>
> I'm planning to do some experiments with the Deca for determining
> phosphorylation sites. The ion mapping methods look promising. Has anyone
> tried mapping phosphorylation sites using the ion mapping function in the
> Deca? Are there any optimization steps that are particularly useful?
>
> On a related note, We currently have the Newojective Picoview set up as
> the
> source, and I think the ion mapping methods require static infusion. How
> do
> other people do infusion with this source?
>
>
> Thanks a lot
>
> Brett
>
>
>
> -------------------------------------
> Brett Phinney, Ph D.
> Department of Biochemistry
> Mass Spectrometry Facility
> Michigan State University
> Phone 517-353-6767
>
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