Dear ABRFers,
I need some advice or references on what you think are the best ways to
make a head to tail cyclized peptide 5mer and 6 mer?
>From my own reading, it seems that a good way is to synthesize the peptide
and cleave it from acid sensitive resin with only the NH2-terminus and the
COOH-terminus free...then cyclize....then deprotect the side chains and
purify the correct product. I've read that highly diluted peptide favors
the cyclized product over polymers.
However, I'm confused by all the cyclic reagents in the literature. Many
people seem to use DPPA which seems to take 5 days at -20 C where as other
labs like to use HBTU, DCC, DIEA or HOBT etc. etc. ....which may take only
an hour.
Can I get a reality check on what is currently the most reliable procedure?
Thanks,
Dick
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