Hi All,
A freind of mine is trying to crystallize an RNA pol III factor, named BRF. The protein has a molecular weight around 12k. It was made via cloned plasmid DNA. Using the same purification method, my friend found the proteins are now having some impurities, which didn't happen before.
The protein has a lighter band near it in gel. I did maldi and found two minor peaks with around 800 Da and 1100 Da smaller than the intact one. We suspect that they are degradation products. Anyone knows which protease might cause this degradation and how it can be avoided? Thanks a lot.
Wei Wu
Department of Chemistry
Michigan State Univ.
517-353-6767
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