Jan-
Any chance that what you are seeing is from a hetero(di/tetra)meric
protein with inter-subunit disulfide bonding, and that the Gly-Cys
represents the C-terminal peptide of the other subunit?
Regards,
John
John Hempel, PhD Ph (412) 624 0161
University of Pittsburgh FAX (412) 624 4759
Department of Biological Sciences
Clapp Hall 301
Pittsburgh PA 15260 USA
email: hempel@psc.edu
http://www.pitt.edu/~biology/faculty/hempel.html
At 02:52 PM 8/14/00 -0400, Jan J. Enghild wrote:
>Dear All,
>
>We have isolated and analyzed a tryptic peptide by Edman degradation
>and MALDI. The peptide contain an internal Cys residue and this
>residue has a Gly-Cys dipeptide added to the side chain (not
>Cys-Gly). Is anyone aware of this modification in other plasma
>proteins or other proteins in general. Thanks in advance.
>
>Jan -
>--
>
>******************************************
> Jan J. Enghild Ph.D.
> Associate Professor
> University of Aarhus, Science Park
> Institute of Molecular
> and Structural Biology
> DK-8000 Aarhus C.
> Denmark
>
> e-mail: jje@mbio.aau.dk
> Telephone: (45) 89-425062
> Fax: (45)89-425063
>*******************************************
>
>
>
>
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