>Hello, every one,
>
>I am currently using silver stain kit (from Bio-Rad) to stain the 2D protein
>gel. However, I always found the background is high (if stain longer) or
>the protein spots are fewer (if stain less time).
>
>Can anyone give me some suggestion for the silver stain procedure for 2D gels.
>
>Thank you very much for your help.
>
>LUO Jun
>luo.32@osu.edu
Blum et al (1987) Electrophoresis, vol 8: pages 103-112.
Dr. Steven P. White
Oklahoma State University
Department of Biochemistry & Molecular Biology
246 NRC
Stillwater, OK 74078
Phone & Voice Mail (405) 744-6191
Fax (405) 744-7799
e-mail swhite@biochem.okstate.edu
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