Luo Jun,
We use a few silver staining methods but generally rely on the method by
Blum et al. as our standard. We have found that methods using a thiosulfate
wash reduce background significantly and all the following use this
approach.
Schevchenko, Wilm, Vorm, Mann, Anal. Chem., 1996, 68, 850-858
Blum, Beier, Gross, Electrophoresis, 1987, 8, 93-99
a third method that I believe is unpublished and the above are all
summarized at the Protana website.
(http://protana.com/services/protocols/default.asp
Best regards,
Lloyd
Lloyd W. Sumner, Ph.D.
Head, Biological Mass Spectrometry
The Samuel Roberts Noble Foundation
PO Box 2180
2510 Sam Noble Parkway
Ardmore, OK 73402
Voice 580-221-7392
Fax 580-221-7380
Email lwsumner@Noble.Org
-----Original Message-----
From: Luo Jun [mailto:luojun@che.eng.ohio-state.edu]
Sent: Sunday, August 20, 2000 7:52 PM
To: Recipients of ABRF List
Subject: Silver stain background for 2DE?
Hello, every one,
I am currently using silver stain kit (from Bio-Rad) to stain the 2D protein
gel. However, I always found the background is high (if stain longer) or
the protein spots are fewer (if stain less time).
Can anyone give me some suggestion for the silver stain procedure for 2D
gels.
Thank you very much for your help.
LUO Jun
luo.32@osu.edu
This archive was generated by hypermail 2b29 : Fri Aug 25 2000 - 08:35:04 EDT