Hi!
I'm a biotechnology student currently taking a traineeship on GMO detection
on foodstuffs and feedingstuffs. Though I applied the PCR technic and some
PCR-based technics (ARMS and RFLPs) in college, I'm new to quantitative PCR
so I have some doubts regarding the instrumentation used in that kind of
assays.
I'm not expecting a too big amount of samples but I need an instrument that
can detect the emission wavelenghts from 2 hybridization fluorogenic probes
at the same time (so I can look for the specific sequence and have a
positive control whithin each reaction tube to monitor amplification
efficiency and avoid false negative results).
That I know of, there's only 3 devices for such determinations: the ABI
PRISM 7700 from Perkin Elmer, the LightCycler from Roche and the iCycler
from Bio-Rad (the ABI PRISM 6700 Automated Nucleic Acid Workstation goes way
beyond our needs). The literature on the LightCycler site says the
LightCycler produces results with smaller variation than the ABI PRISM 7700,
but I've heard that the latter is better than the Roche's instrument.
Besides I prefer the one probe approach used on the TaqMan probes for the PE
equipments and those are not recomended for use in the LightCycler...
Finally, I never heard anything about the iCycler from Bio-Rad -- is it
because it's new or because it's no good?
So, what I want is opinions about the equipments designed to perform
quantitative PCR currently available. I need to know what instrument should
I recomend my traineeship manager to buy.
If anyone can help me on this instrumentation "mess", I'd be greatly
thankful...
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