Re: trypsin activity in guanidine hydrochloride

From: fperini@unmc.edu
Date: Tue Aug 22 2000 - 12:58:53 EDT

Next message: mcada002@mc.duke.edu: "Replace HP1090?"
Previous message: Satya Yadav, Ph.D.: "Hello everyone,"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

I do not like to use guanidine,because it affects trypsin in more than one
way.I had some
success by denaturing in 6M guanidine,and then diluting about 10x,and
adjusting the pH,
prior to addition of trypsin.This method is not good if your sample is
small,and you end
up with a large final volume.

Fulvio Perini,Ph.D.
986805 Nebraska Med.Cntr.
Omaha,NE 68198-6805
ph:402-559-6184/FAX 402-559-4651
fperini@unmc.edu

Next message: mcada002@mc.duke.edu: "Replace HP1090?"
Previous message: Satya Yadav, Ph.D.: "Hello everyone,"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

This archive was generated by hypermail 2b29 : Fri Aug 25 2000 - 08:35:04 EDT