Re: Pep Syn

From: fperini@unmc.edu
Date: Tue Aug 22 2000 - 18:23:41 EDT


I do not think that the synthesis should be such a problem.The residues are
not basic
until deprotected,and having only two adjecent Arg should pose no
problem.The deprotection
may take longer than usual,depending on the protecting group.

Rebecca Ettling <ettlingr@musc.edu>@aecom.yu.edu> on 08/22/2000 12:09:24 PM

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Subject: Pep Syn

Hello everyone,

Does anyone have any insights as to whether this peptide:

RRPKG RGKRR RENQR PTDCHL

will be a straight forward synthesis or a difficult one ?

I use Fmoc chemistry with HBTU activation. I am worried about the
synthesis of so many basic residues in very close proximity. If anyone
has any helpful tips, I woud appreciate them very much.

As always, thank you in advance for your advice.

-- Rebecca P. Ettling
Biotechnology Resource Laboratory
Protein Sequencing and Peptide Synthesis Facility
Medical University of South Carolina
173 Ashley Avenue, Room 733D BSB
Charleston, SC 29403
Tel.: (843) 792-1271
Fax: (843) 792-1264

If I have seen farther than others, it is because I was standing on the
shoulders of giants.--Isaac Newton



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